Super-resolution imaging comprises a number of techniques that overcome the diffraction limit of a typical optical microscope in order to obtain high-resolution images. Within this toolkit of techniques, SMLM is one example.
SMLM typically relies upon a CCD or CMOS camera to collect the fluorescence or photoluminescence emission from a sample of blinking particles whose reversible transitions between an activated state and a dark state aid in resolving closely-packed multiple emitters within the sample.
Our group has recently developed a detection scheme for a single-molecule localization microscope incorporating four single-photon avalanche photodiode (SPAD) detectors and a time-correlated single-photon counter (TCSPC), which was demonstrated by creating a spatial mapping of quantum dot positions based on emission intensity.
